软骨素酶AC 来源于肝素黄杆菌

4.2.2.5 ( BRENDA | IUBMB )

MFCD00130796

NA.54

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Contains potassium phosphate buffer salts and BSA as stabilizer.
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One unit will cause a ΔA₂₃₂ of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 ℃. Reaction volume: 3.1 ml (light path 1 cm).

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

lyophilized powder

200

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Protein, ~15% Lowry

0.02 M phosphate buffer: soluble (pH 7.0)

Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount

−20℃

5 units in serum bottle

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)