Chondroitinase B from Flavobacterium heparinum

MFCD00166444

NA.54

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.
The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 ℃ and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co²⁺, Fe³⁺ and Ba²⁺ also inhibit its activity.

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25℃ using chondroitin sulfate B as substrate.

200

lyophilized powder (with BSA as stabilizer)

dry ice

−20℃

GHS07

Warning

H319

P264 - P280 - P305 + P351 + P338 - P337 + P313

Eye Irrit. 2

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)