CMP-Sialic Acid Synthetase from Neisseria meningitidis group B

2.7.7.43 ( BRENDA | IUBMB )

MFCD01323074

NA.54

Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.

The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.

Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg²⁺ is essential for the catalytic functionality of CMP-sialic acid synthetase.

One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 ℃ at pH 8.0.

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 ℃ for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).

200

expressed in E. coli BL21

lyophilized solid

≥10 units/mg protein

26.0 kDa

dry ice

−20℃

13 - Non Combustible Solids

WGK 3