您的位置:首页 > 产品中心 > CHIRAL-HSA 高效液相色谱柱
CHIRAL-HSA 高效液相色谱柱
CHIRALPAK® HSA HPLC Column
产品别名
CHIRAL-HSA 高效液相色谱柱
CHIRALPAK® HSA HPLC Column
基本信息
eCl@ss | 32110501 |
NACRES | SB.52 |
General description【一般描述】 | CHIRALPAK HSA, which uses human serum albumin as the chiral selector, is highly selective for acidic racemates, preferably weak and strong acids, zwitterionic and non-protolytic (neutral) compounds. Phosphate buffers (normally 0.01-0.1M, pH 5-7) with addition of organic modifiers are used as mobile phases. Enantioselectivity and retention can be regulated by changing the mobile phase composition. However, the primary use of CHIRALPAK HSA is for fast drug/protein binding studies (1). To calculate the % protein binding, measure the retention time of an unretained compound (t0) and the compound of interest (tr) on the CHIRALPAK HSA column. Then use the capacity factor equation: k = (tr - t0)/tr to calculate the % protein binding (P): P = 100k/(k+1) Different types of mobile phases can be used. A mobile phase consisting of 6% 2-propanol in 20 mM potassium phosphate buffer, pH 7.0 gives data in good agreement with literature data. The mobile phase conditions should be chosen to suit the drugs to be tested, i.e., for high protein binding drugs a mobile phase with higher eluting strength might be needed in order to reduce retention times. (1) Goodman, A.; Gilman, A.G. The Pharmacological Basis of Therapeutics, 9th Edition, McGraw-Hill: New York, 1996; pp 1712-1792. |
Recommended products【推荐产品】 | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
Legal Information【法律信息】 | CHIRALPAK is a registered trademark of Daicel Corp. |
产品性质
material【物料】 | stainless steel column |
Quality Level【质量水平】 | 100 |
agency | suitable for USP L79 |
product line【产品线】 | CHIRALPAK® |
packaging【包装】 | pkg of 1 ea |
manufacturer/tradename | CHIRALPAK® |
parameter【参数】 | 137 bar pressure (2000 psi) 20-30 ℃ temperature 40 ℃ max. temp. |
technique(s) | HPLC: suitable |
L × I.D.【长度 × 内径】 | 10 cm × 2.1 mm |
matrix【基质】 | fully porous particle |
matrix active group【基质活性基团】 | human serum albumin phase |
Particle size【粒径】 | 5 μm |
operating pH | 2-8 |
separation technique【分离技术】 | chiral |