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FRASER Listeria Ammonium iron (III) Supplement

产品编号:4060833
规格:for Listeria spp., pkg of 10 vials, for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base)
CAS NO:1185-57-5
包装规格:10 VIAL
产品类别:进口试剂
品牌:Millipore
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
406083310 VIAL918
产品别名

1185-57-5

FRASER Listeria Ammonium iron (III) Supplement

Listeria selective supplement

FRASER Listeria Ammonium iron(III) Supplement

基本信息
Empirical Formula【经验(实验)分子式】
C6H8O7 · xFe · xH3N
MDL number
MFCD03099410
EC Index Number【EC索引号】
214-686-6
General description【一般描述】
For preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement and FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., and together with esculin (a component of the FRASER broth base), permits the β-D-glucosidase detection in Listeria spp.
Application【应用】
FRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.
Linkage【联系】
Replaces: 1000920017
Analysis Note【分析说明】
Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): ≥ 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic
Legal Information【法律信息】
ALOA is a registered trademark of Biolife Italiana S.r.l.
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
200
agency
ISO 11290
ISO
form【形式】
lyophilized powder
potency【效能】
>2000 mg/kg LD50, oral (Rat)
composition【组成】
Ammonium iron (III) citrate, 250 mg per vial
packaging【包装】
pkg of 10 vials
technique(s)
microbiological culture: suitable
pH【pH值(酸碱度)】
6-8 (20 ℃, 100 g/L in H2O)
solubility【溶解性】
1200 g/L
application(s)
food and beverages
microbiology
pathogen testing
compatibility【相容性】
GranuCult® FRASER Broth (Base) (1.10398)
GranuCult® Half FRASER (Demi FRASER) Broth (Base) (1.00025)
storage temp.【储存温度】
15-25℃
suitability【适用性】
Listeria spp.
InChI
1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3
InChI key
FRHBOQMZUOWXQL-UHFFFAOYSA-K
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 1
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