KAPA SYBR^® FAST 一步法

NA.55

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol.

KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV reverse transcriptase, RNase inhibitor, and a novel DNA polymerase evolved via directed evolution.

KAPA SYBR^® FAST One-Step is suitable for:

• One-step quantitative reverse transcriptase PCR (qRT-PCR)
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• miRNA research
• Gene knockdown validation

KAPA SYBR^® FAST DNA Polymerase displays no enzymatic activity at ambient temperature. This prevents the formation of misprimed products and primer-dimers during reaction setup prior to the first denaturation step and results in high PCR specificity and accurate quantification. Moloney murine leukemia virus reverse transcriptase (M-MuLV-RT) has a high affinity for RNA and is optimized for cDNA synthesis at 42℃. The RNase Inhibitor safeguards against degradation of RNA target due to RNase contamination.

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA SYBR FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20℃. The KAPA RT Mix is temperaturesensitive, and should be stored at -20℃ and kept on ice during use.

The SYBR Green I dye contained in the KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30  freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20℃ when not in use. When protected from light, reagents are stable in the dark at 4℃ for at least one week and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

For Research Use Only. Not for use in diagnostic procedures.

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

组份不可单独销售
qPCR Master Mix (2X) with ROX^™ High incorporated
dUTP
50X KAPA RT Mix

100

sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions

≤6 mo.

dNTPs included: no
hotstart: no

kit of 1 mL (100 x 20 μL rxn; KK4660)
kit of 10 mL (1000 x 20 μL rxn; KK4662)
kit of 5 mL (500 x 20 μL rxn; KK4661)
pkg of

Roche

2 ×

RT-PCR: suitable
qPCR: suitable

purified DNA

for use with Applied Biosystems^® 5700
for use with Applied Biosystems^® 7000
for use with Applied Biosystems^® 7300
for use with Applied Biosystems^® 7700
for use with Applied Biosystems^® 7900HT
for use with StepOne^™
for use with StepOnePlus^™

SYBR^® Green

dry ice

−20℃

GHS08

Warning

H371

P260 - P264 - P270 - P308 + P311 - P405 - P501

STOT SE 2

12 - Non Combustible Liquids

WGK 1