BCECF/AM-CAS 117464-70-7-Calbiochem

MFCD00036969

A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.
A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.

Cell permeable: yes
Primary Target
Monitor intracellular pH changes
Product does not compete with ATP.
Reversible: no

Toxicity: Irritant (B)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.

Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥85% (mixture of isomers, HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

white to off-white

acetonitrile: 25 mg/mL
DMF: soluble
DMSO: soluble

λ_ex 500-504 nm
λ_em 525-531 nm

ambient

−20℃

1 mg in Glass bottle
Packaged under inert gas

11 - Combustible Solids

WGK 3