Biotin, Fluorescein Conjugate

100

lyophilized solid

Calbiochem^®

OK to freeze
protect from light

orange

DMF: 5 mg/mL
DMSO: 5 mg/mL
methanol: 5 mg/mL

ambient

−20℃

1 mg in Plastic ampoule

Avidin (and streptavidin) has 4 binding sites for biotin. Taking advantage of the high affinity between avidin and biotin, this fluorescein-conjugated biotin-X (a 6-atom spacer) provides a method for fluorescent detection of biotinylated antibodies.

EIA (1:1,000)

Tissue Staining (1:50 - 1:100)

Toxicity: Standard Handling (A)

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Make a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Gruber, H.J., et al. 1998. Biochim. Biophys. Acta1381, 203

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1