Calpain-1 Substrate II, Fluorogenic-Calbiochem

C₇₂H₉₇N₁₇O₁₆S

1488.71

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.
An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.

Primary Target
Calpain-1 Substrate
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Irritant (B)

H₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H

Supplied as a trifluoroacetate salt.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Cuerrier, D., et al. 2007. J. Biol. Chem.282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem.280, 40632.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥97% (HPLC)

lyophilized solid

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

red

DMSO: 5 mg/mL

λ_ex ~505 nm
λ_em ~335 nm

wet ice

−20℃

1 mg in Glass bottle
Packaged under inert gas

11 - Combustible Solids

WGK 1