NFF-2-Calbiochem

C₇₉H₁₀₅N₁₉O₁₉S

1656.86

A quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 59,400 M^-1s^-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 54,000 M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 55,300 M^-1s^-1).
A quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 5.9 x 10⁴ M^-1sec^-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 5 x 10⁴ M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 5.5 x 10⁴ M^-1s^-1).

kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)
Product does not compete with ATP.
Primary Target
Matrix Metalloproteinases (MMPs)
Reversible: no
Cell permeable: no

Toxicity: Standard Handling (A)

MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)

Supplied as a trifluoroacetate salt.

Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

lyophilized solid

Calbiochem^®

OK to freeze
desiccated
protect from light

yellow

DMSO: 5 mg/mL

λ_ex ~325  nm
λ_em ~393 nm

ambient

−20℃

1 mg in Plastic ampoule

11 - Combustible Solids

WGK 1