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Calpain Substrate III, Fluorogenic-Calbiochem
| 产品编号： | 4061800 |
| 规格： | A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2. |
| 包装规格： | 1 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Calpain Substrate III, Fluorogenic-Calbiochem
(DABCYL)-TPLK~SPPPSPR-(EDANS)
基本信息
| Empirical Formula【经验(实验)分子式】 | C80H114N20O18S |
| Molecular weight | 1675.95 |
| General description【一般描述】 | A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC and α-spectrin cleavage site-based substrate. Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate. A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intact Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate. |
| Biochem/physiol Actions【生化/生理作用】 | Product does not compete with ATP. kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and <0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively Primary Target Substrate sequence that is optimized for calpain-1 and -2 Reversible: no Cell permeable: no |
| Warning【警告】 | Toxicity: Irritant (B) |
| Sequence【序列】 | DABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS |
| Physical form【外形】 | Supplied as a trifluoroacetate salt. |
| Reconstitution【重悬】 | Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. |
| Other Notes【其他说明】 | Tompa, P., et al. 2004. J. Biol. Chem.279, 20775. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| Assay【测定】 | ≥98% (HPLC) |
| form【形式】 | lyophilized solid |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze protect from light |
| color【颜色】 | red |
| solubility【溶解性】 | water: 1 mg/mL DMSO: 5 mg/mL |
| fluorescence【荧光】 | λex 320 nm λem 480 nm |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 1 mg in Plastic ampoule Packaged under inert gas |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
