TIRAP Inhibitor Peptide, Cell-Permeable-Calbiochem

C₁₆₃H₂₆₈N₅₂O₃₈S

3596.26

A synthetic, cell-permeable peptide that corresponds to mouse toll-interleukin 1 receptor (TIR) domain-containing adapter protein 138-151 (TIRAP; also called Mal (MyD88-adapter-like), fused to the Drosophila antennapedia sequence. Shown to specifically inhibit LPS-induced, but not CpG-induced, NF-κB activation, PKR phosphorylation, and JNK phosphorylation in RAW.κB cells at ~40 µM. Also reported to block IκBα degradation.
A cell-permeable synthetic peptide containing mouse TIRAP_138-151 fused to the Drosophila Antennapedia sequence. Specifically inhibits LPS-, but not CpG-induced NF-κB activation, PKR phosphorylation, and JNK phosphorylation in RAW.κB cells at ~40 µM. Also reported to block IκBα degradation.

Product does not compete with ATP.
Primary Target
LPS-induced NF-κB activation, PKR phosphorylation, and JNK phosphorylation in RAW.kB cells
Cell permeable: yes
Reversible: no

Toxicity: Standard Handling (A)

H-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Leu-Gln-Leu-Arg-Asp-Ala-Ala-Pro-Gly-Gly-Ala-Ile-Val-Ser-OH

Supplied as a trifluoroacetate salt.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Toshchakov, V., et al. 2002. Nat. Immunol.3, 392.
Fitzgerald, K.A., et al. 2001. Nature413, 78.
Henneke, P. and Golenbock, D.T. 2001. Nat. Immunol.2, 828.
Horng, T., et al. 2001. Nat. Immunol.2, 835.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥97% (HPLC)

lyophilized solid

Calbiochem^®

OK to freeze
desiccated (hygroscopic)
protect from light

white

DMSO: 5 mg/mL

wet ice

−20℃

1 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 1