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产品性质
| biological source【生物来源】 | human colon |
| description【描述】 | Human columnar cell adenocarcinoma of the colon |
| growth mode【生长模式】 | Adherent |
| morphology【形态学】 | Epithelial; cells grow as floating and loose aggregates with some adherent cells. |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Description【细胞系描述】 | LIM2550 is derived from an adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0169. |
| Culture Medium【培养基】 | RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM. |
| Subculture Routine【传代培养常规】 | Optimal split ratio 1:4-1:8 (seeding density 1-2 x 104 cells/cm2). Cells incubated at 37℃ with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37℃ for 5 min. LIM2550 requires the growth medium to be changed 3 times each week. Feed and passage the cells as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding). |
| Other Notes【其他说明】 | Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
