您的位置：首页 > 产品中心 > SIGi001-A-11
产品性质
| biological source【生物来源】 | human |
| reprogramming method【重编程方法】 | retrovirus |
| description【描述】 | age (N/A) |
| manufacturer/tradename | EBiSC™ |
| gender【性别】 | female |
| growth mode【生长模式】 | adherent (pluripotent) |
| technique(s) | cell culture | stem cell: suitable |
| relevant disease(s)【相关疾病】 | corticobasal degeneration |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| General description【一般描述】 | Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop. The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching. |
| Cell Line Origin【细胞系来源】 | Depositor sigma-aldrich |
| Cell Line Description【细胞系描述】 | Derivation Primary cell type: - Reprogramming method Vector type: Integrating Vector: Virus Virus type: Retrovirus Gene list: KLF4 MYC POU5F1 SOX2 Have the reprogramming vectors been silenced: Yes Merthods used: rtpcr Xeno free conditions: no Derived under gmp: no Available as clinical grade: no Characterization Analysis of Undifferentiated Cells Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-1 No SSEA-4 Yes TRA 1-60 Yes POU5F1 (OCT-4) Yes Differentiation potency Ectoderm: Ectoderm In vitro spontaneous differentiation Marker Expressed HES5 Yes NeuroD1 Yes PAX6 Yes Endoderm: Endoderm In vitro spontaneous differentiation Marker Expressed CXCR4 Yes GATA6 Yes SOX17 Yes Mesoderm: Mesoderm In vitro spontaneous differentiation Marker Expressed MIXL1 Yes NCAM1 Yes VIM Yes Microbiology / Virus Screening HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation Genotyping Karyotyping Passage number: P37 Cell line karyotype: 46,XX[17]/47,XX,+12[2] Karyotyping method: G-Banding STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase. Genetic Modification Disease/phenotype related modifications Disease: Corticobasal degeneration There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms Type of modification: Isogenic Gene: MAPT Chromosome location: 17q21.31 Nucleotide sequence HGSV: NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]] Target locus modification: Mutated Description: P301S+IVS10+16 C>T; Regarding homozygosity/heterozygosity- Disease: Progressive supranuclear palsy There are three disease associations for the edited subclone: PSP (progressive supranuclear palsy) /CBD (Corticobasal degeneration) / FTDP-17 (Frontotemporal dementia and parkinsonism linked to chromosome 17) - like symptoms Type of modification: Isogenic Gene: MAPT Chromosome location: 17q21.31 Nucleotide sequence HGSV: NM_016834.4 : c.[ c.727 >T; c.[741+16C>T]+[741+16C>T]] Target locus modification: Mutated Description: P301S+IVS10+16 C>T; Regarding homozygosity/heterozygosity- |
| Linkage【联系】 |
Note: EAUA and CLIP must be completed before order fulfillment |
| Subculture Routine【传代培养常规】 | Medium: mTeSR® Passage method: EDTA Matrix: Matrigel® / Geltrex® CO2 concentration: 5% O2 concentration: 21% Temperature: 37℃ |
| Legal Information【法律信息】 | EBiSC is a trademark of Fraunhofer-Gesellschaft GELTREX is a registered trademark of Life Technologies Corporation Matrigel is a registered trademark of Corning, Inc. mTeSR is a registered trademark of WiCell Research Institute, Inc. |
安全信息
| Storage Class Code【储存分类代码】 | 6.2 - Infectious substances |
| WGK | WGK 3 |
