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T47D/TR-1

产品编号:4062834
规格:from human breast, 16022513
包装规格:1 VIAL
产品类别:进口试剂
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40628341 VIAL10560
产品性质
biological source【生物来源】
human breast
growth mode【生长模式】
Adherent
morphology【形态学】
polygonal epithelia
technique(s)
cell culture | mammalian: suitable
shipped in【运输】
dry ice
storage temp.【储存温度】
−196℃
基本信息
General description【一般描述】
T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 cells are growth inhibited by fulvestrant. Oestrogen receptor.
Cell Line Description【细胞系描述】
The T47D/TR-1 is a breast cancer cell line resistant to tamoxifen. The T47D/TR-1 cell line has been established by long term treatment of T47D/S2 cells with 1 μM tamoxifen. Clonal selection was performed in medium without tamoxifen. After growth in presence of 1 μM tamoxifen for 10 months, the growth rate was similar to the parental cells.
Culture Medium【培养基】
RPMI 1640 w/o Phenol Red (R7509) + 2% FBS (F2442) + 2.5 mM Ala-Gln (G8541) + 8μg/ml Insulin (I9278)
Subculture Routine【传代培养常规】
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37℃. Recommend media changes every 2-3 days.
Other Notes【其他说明】
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Citation【引用】
If use of this culture results in a scientific publication, it should be cited in the publication as: T47D/TR-1 (ECACC 16022513)
产品说明
Disclaimer【免责声明】
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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