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产品性质
| biological source【生物来源】 | human dermis (fibroblast) |
| reprogramming method【重编程方法】 | episomal |
| description【描述】 | age (30-34) |
| manufacturer/tradename | EBiSC™ |
| gender【性别】 | male |
| growth mode【生长模式】 | adherent (pluripotent) |
| technique(s) | cell culture | stem cell: suitable |
| relevant disease(s)【相关疾病】 | none |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| General description【一般描述】 | Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop. The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching. |
| Cell Line Origin【细胞系来源】 | Depositor Klinikum der Universität zu Köln |
| Cell Line Description【细胞系描述】 | Derivation Primary cell type: Fibroblast of dermis Location of primary tissue procurement: Zone of skin Tissue collection year: 2016 Passage number reprogrammed: 3 Reprogramming method Vector type: Non-integrating Vector: Episomal Is the reprogramming vector detectable: No Merthods used: pcr Notes on reprogramming vector detection: episomal vector cassette absent Files showing reprogramming vector expressed or silenced: Image / Result Xeno free conditions: no Derived under gmp: no Available as clinical grade: no Characterization Analysis of Undifferentiated Cells Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles CD30 Yes - TRA 1-80 Yes - - DNMT3b Yes - CD90 Yes - SSEA-4 Yes - - SSEA-1 Yes - NANOG Yes - - OCT 4 Yes - - FOXD3 Yes - SOX2 Yes - EpiPluriScore: Score: 0,24 Marker Present Absent mCpG OCT4 Report: Epi_Pluri_score_2017.jpg Epi_pluri_score_result_20170621.pdf - Analysis Report Epi Pluri Score Morphology: hiPS NP0105-19 before banking and after thawing hiPS NP0105-19 on different days post thaw Differentiation potency Ectoderm: Ectoderm In vitro directed differentiation Marker Expressed Nestin No Endoderm: Endoderm In vitro directed differentiation Marker Expressed SOX17 Yes Morphology: Mesoderm: Mesoderm In vitro directed differentiation Marker Expressed BRACHYURY Yes Morphology: Microbiology / Virus Screening HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Genotyping Karyotyping Passage number: 15 Cell line karyotype: Chr07: Larger duplication (20Mbp) mosaic (80%) on long arm. Karyotyping method: Molecular karyotyping by SNP array Image / Result |
| Linkage【联系】 |
Note: EAUA and CLIP must be completed before order fulfillment |
| Subculture Routine【传代培养常规】 | Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: other Culture base medium: StemFlex™ Basal Medium Main protein source: - Passage Number banked (pre-EBiSC): 21 No. Vials banked (pre-EBiSC): 25 |
| Legal Information【法律信息】 | EBiSC is a trademark of Fraunhofer-Gesellschaft StemFlex is a trademark of Life Technologies Corporation |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
