羟乙基哌嗪乙硫磺酸

C₈H₁₈N₂O₄S

238.30

883043

MFCD00006158

HEPES has been described as one of the best all-purpose buffers available for biological research.[7] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[1]

HEPES has been used:

• As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo†
• As a component of Hanks′ solution used in the degradation testing of Iron†
• As a component of HEPES medium in the transfection of cell culture with plasmids†
• Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells†
• As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies†

200

synthetic

reagent grade

≥99% (titration)

crystalline

6.8 - 8.2

7.5

OCCN1CCN(CC1)CCS(O)(=O)=O

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

JKMHFZQWWAIEOD-UHFFFAOYSA-N

11 - Combustible Solids

WGK 1