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RealSeq®-AC
| 产品编号： | 4063209 |
| 规格： | NGS library preparation kits for miRNAs and small RNAs, For total RNA samples from tissue or cells |
| 包装规格： | 1 KIT |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| shipped in【运输】 | wet ice |
基本信息
| General description【一般描述】 | RealSeq® is a novel method for preparing small-RNA sequencing libraries that reduces incorporation bias in Next-Generation-Sequencing (NGS). Most bias stems from sequence-dependent variability in the enzymatic ligation reactions that attach the two adapters to the 3′ and 5′ ends of the miRNAs/small RNAs during preparation of sequencing libraries. By using a novel single adapter and circularization, RealSeq® greatly reduces library preparation bias. This technology solves the problem of commonly used sequencing library preparations that lead to underdetection of many miRNAs. RealSeq®-AC is a small RNA library preparation kit compatible with Illumina Next Generation Sequencing (NGS) technology. RealSeq®-AC is optimized for RNA input between 1ng and 1000ng of total RNA from tissue or cells. |
| Application【应用】 | This kit is for small RNA library preparation for NGS. RNA-Seq for small RNA and miRNA; NGS library generation for small RNA and miRNA; RealSeq®-AC is highly efficient, detecting more miRNAs in total RNA samples. Application Note RealSeq® accurately quantifies small RNA sequenced on the Illumina Platform. RealSeq®-AC uses ligation of a single adapter producing small RNA libraries with more miRNAs compared to library prep kits using two adapters. |
| Features and Benefits【特点和优势】 |
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| Components【组分】 | This kit is for 12 libraries. This product is comprised of the following: RNA buffer RealSeq Adapter RNAse inhibitor Ligation buffer Ligase Blocking Agent Blocking Enzyme Blocking Buffer RealSeq Enzyme RealSeq Buffer Dimer Removal Agent RT Primer dNTPs RT buffer RT Enzyme PCR Buffer PCR Polymerase Rnase Free Water RealSeq Beads Reverse Index Primers* Forward Primer miRNA Control Adapter Dilution Buffer * Index Primers 1-12 are included in REALSEQAC-1KT (500-00012) See experimental protocol for more information. |
| Legal Information【法律信息】 | RealSeq is a registered trademark of SomaGenics, Inc. |
产品说明
| Storage and Stability【储存及稳定性】 | The three components should be stored at the specified Temperature upon arrival of the Kit.
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安全信息
| Pictograms【象形图】 | GHS09 |
| Hazard Statements | H411 |
| Precautionary Statements | P273 |
| Hazard Classifications【危险分类】 | Aquatic Chronic 2 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
