Amphiregulin human

MFCD00282118

NA.32

Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.

Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.

This product should be stored at -20℃. After preparation, the product can be stored at 2-8℃ for one month, or frozen in aliquots at -20℃ or -70℃ for more extended storage.

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.

It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED₅₀ for this effect is typically 5-15 ng/mL.

100

human

expressed in E. coli

≥97% (SDS-PAGE)

lyophilized powder

10-100 ng/mL ED₅₀/EC₅₀

~11 kDa

pkg of 1 mg

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

cell culture | mammalian: suitable

endotoxin, tested

ketatinocyte, KAF, recombinant human

P15514

−20℃

human ... AREG(374)

13 - Non Combustible Solids

WGK 3

Eyeshields, Gloves, type N95 (US)