羧基酯酶2 人

3.1.1.1 ( BRENDA | IUBMB )

NA.54

Carboxylesterase 2 human gene is mapped to human chromosome 16q22.1.
Carboxylesterase belongs to the α/β hydrolase fold family and is involved in drug metabolism and activation. It is present in colon, liver, small intestine, heart, brain and testis. The hydrolase activity of small intestine is attributed to carboxylesterase 2 human. It has two glycosylation sites at 103 and 267 residues with molecular mass 60kDa.

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.
Carboxylesterase 2 human has been used:

• in the in vitro enzyme-based inhibitor screening assay
• in substrate selectivity assay with emission ratiometric two-photon probe (SE1)
• as a reference standard in the enzyme assay with various 4-nitrophenyl and 1-naphthyl based substrates

Carboxylesterase 2 may play a key role in biotransformation of a variety of ester containing drugs and prodrugs.

Carboxylesterase 2 human identifies the site containing a large alcohol and small acyl group. It shows less transesterification activity due to presence of conformational interference in active site.
可水解酯和酰胺键的丝氨酸酯酶家族成员。羧基酯酶2是一种内质网结合的水解酶，在异源生物解毒和活化含酯治疗剂中起关键作用。羧基酯酶2也可参与海洛因和可卡因等药物的解毒过程。该酶被认为在脂质代谢中起作用。

One unit will produce 1 nanomole of 4-nitrophenol from 4-nitrophenyl acetate per minute at pH 7.4 at 37 deg C.

200

expressed in baculovirus infected BTI insect cells

liquid

≥500 units/mg protein

60 kDa

(0.5 ml)

dry ice

−70℃

12 - Non Combustible Liquids

nwg