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Aconitase Assay Kit
产品性质
| usage【用途】 | sufficient for 100 colorimetric tests |
| Detection Method | colorimetric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | Suitable for aconitase activity determination in biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) |
基本信息
| General description【一般描述】 | Aconitase (Aconitate Hydratase) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich′s ataxia and diabetes. |
| Features and Benefits【特点和优势】 | Compatible with high-throughput handling systems. |
| Principle【原理】 | The Aconitase Assay Kit measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity. |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H334 |
| Precautionary Statements | P261 - P284 - P501 |
| Hazard Classifications【危险分类】 | Resp. Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
