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Duolink® flowPLA Mouse/Rabbit Starter Kit-Far Red
| 产品编号： | 4066705 |
| 规格： | Duolink® PLA kit for Flow Cytometry with Far Red Detection and Mouse/Rabbit probes |
| 包装规格： | 1 KIT |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| Quality Level【质量水平】 | 200 |
| technique(s) | flow cytometry: suitable immunofluorescence: suitable proximity ligation assay: suitable |
| fluorescence【荧光】 | λex 644 nm; λem 669 nm |
| suitability【适用性】 | suitable for fluorescence |
| storage temp.【储存温度】 | −20℃ |
基本信息
| Specificity【特异性】 | Far Red Fluorescence Detection Reagents Use appropriate laser for λex 644 nm excitation Use appropriate filter for λem 669 nm emission. Application Note Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended. Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects. View full Duolink® product list |
| Application【应用】 | Based on proximity ligation assay (PLA), the Duolink® PLA technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells. Duolink® flowPLA Starter Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest and the Duolink® flowPLA starter Kit. The flowPLA Starter Kits are available with 3 different fluorophores: Violet, Green, and FarRed. The flowPLA starter Kits contain all the necessary reagents reagents for 100 Duolink® PLA reactions, including a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS) and all the reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, (rabbit and mouse-raised) primary antibodies, and corresponding PLA Probes. Follow the Duolink® PLA Flow Cytometry Protocol to use this product. Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more. |
| Features and Benefits【特点和优势】 |
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| Components【组分】 | Duolink® flowPLA Detection Kit – Far Red DUO94004-40TST Duolink® In Situ PLA® Probe Anti-Mouse MINUS DUO92004-40TST Duolink® In Situ PLA® Probe Anti-Rabbit PLUS DUO92002-40TST Duolink® In Situ Wash Buffer, Brightfield (DUO82047-4L) See datasheet for more information. |
| Principle【原理】 | Duolink® flow PLA Mouse/Rabbit Starter Kit - Far Red has been used in the proximity ligase assay (PLA) to detect co-localization of human immune deficiency virus (HIV) protein, negative factor (Nef) with serine incorporator (SERINC3(iHA)) in human T-lymphoblastoid CEM A*02 cells. |
| Legal Information【法律信息】 | Duolink is a registered trademark of sigma-aldrich Co. LLC PLA is a registered trademark of sigma-aldrich Co. LLC Plexiglas is a registered trademark of Arkema |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
