您的位置：首页 > 产品中心 > Pyrophosphate (PPi) Assay Kit (Fluorometric/Colorimetric)
Pyrophosphate (PPi) Assay Kit (Fluorometric/Colorimetric)
| 产品编号： | 4068448 |
| 规格： | sufficient for 50 colorimetric or 100 fluorometric tests |
| 包装规格： | 1 KIT |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| Quality Level【质量水平】 | 100 |
| web metadata keyword.default | Pyrophosphate (PPi) Assay Kit (Fluorometric/Colorimetric)|sigma-aldrich |
| Detection Method | colorimetric fluorometric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | The kit is suitable for the determination of PPi concentration, monitoring pyrophosphate release by a variety of enzymes and for the screening inhibition or activities of enzymes that consume pyrophosphate. Suitable samples include plasma, serum and other biological fluids, cell culture extracts and tissue lysates. |
基本信息
| General description【一般描述】 | Inorganic pyrophosphate (diphosphate, pyrophosphoric acid or PPi) is a small molecule formed during, or required as a substrate, for a number of biochemical reactions. Enzymatic reactions that generate PPi include ATP hydrolysis, DNA and RNA polymerization, cyclic AMP formation, and enzymatic activation of fatty acids to form their CoA esters. Conversely, enzymes that utilize PPi may include cyclases, hydrolases and ligases. Measurement of inorganic pyrophosphate (PPi) in plasma, serum and other biologic fluids is important in studies of bone metabolism, renal stone disease, and certain types of arthritis. |
| Features and Benefits【特点和优势】 | The Pyrophosphate (PPi) Assay Kit is:
|
| Principle【原理】 | The Pyrophosphate (PPi) Assay Kit provides a fast, convenient and ultrasensitive method for determination of free inorganic pyrophosphate levels in biological material. PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (λEx = 535 nm/λEm = 587 nm) or color (A570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay. |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H317 - H334 |
| Precautionary Statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Classifications【危险分类】 | Resp. Sens. 1 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
