Alkaline Phosphatase Activity Fluorometric Assay Kit

100

Alkaline Phosphatase Activity Fluorometric Assay Kit|sigma-aldrich

fluorometric

−20℃

The kit is suitable for the measurement of alkaline phosphatase activity in serum, plasma, urine, semen, cell culture media, cells and tissue.

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. The change in alkaline phosphatase level and activity is associated with several diseases in the liver and bones. Alkaline phosphatase is also a popular enzyme conjugated to secondary antibody in ELISA assays.

Alkaline Phosphatase Activity Fluorometric Assay Kit:

• Compatible with high-throughput handling systems.
• Simple procedure with results in ∼1 hour.
• Ultra-sensitive, with a detection sensitivity of ∼1 μU.

In the Alkaline Phosphatase Activity Fluorometric Assay Kit, ALP cleaves the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate resulting in an intense fluorescent signal (λ_Ex = 360 nm/λ_Em = 440 nm).

10 - Combustible liquids