Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse

NA.46

A soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase. The product is a soluble complex of calf intestinal alkaline phosphatase and mouse monoclonal antibodies to alkaline phosphatase.
Monoclonal Anti-Alkaline Phosphatase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in mouse has been used in:

• Immunohistological analysis
• Immunohistochemistry assay
• Immunocytochemical staining

Apoptosis of β-cells in mouse pancreatic sections was analyzed by incubating section with TUNEL and anti-insulin antibody. APAAP was used as the secondary antibody and BCIP/NBT (Sigma) as the substrate.

Mouse Monoclonal Alkaline Phosphatase Anti-Alkaline Phosphatase is a soluble complex formed from levamisole-resistant calf intestinal alkaline phosphatase and purified mouse monoclonal antibody to alkaline phosphatase. The use of a mouse monoclonal alkaline phosphatase anti-alkaline phosphatase (APAAP) complex, in conjunction with a primary mouse antibody and a secondary bridging antibody (anti-mouse IgG), results in an intense signal with a very low background, while avoiding problems inherent to the covalent conjugation of antibodies. The technology of unlabelled antibody peroxidase-anti peroxidase (PAP) and the alkaline phosphatase anti- alkaline phosphatase (APAAP) method has applications in immunostaining. APAAP monoclonal antibodies complex are homogenous and this method is effective and its sensitive.

Solution in 0.05 M Tris buffered saline, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin and 15 mM sodium azide.

200

mouse

purified immunoglobulin

primary antibodies

AP1B9, monoclonal

Concentrate

buffered aqueous solution

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil

IgG1

wet ice

2-8℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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