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Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit
| 产品编号： | 4070665 |
| 规格： | IgG fraction of antiserum, buffered aqueous solution |
| 包装规格： | 1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD00162640 |
| General description【一般描述】 | IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. |
| Immunogen【免疫原】 | purified mouse IgG |
| Application【应用】 | Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution. |
| Biochem/physiol Actions【生化/生理作用】 | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization. |
| Physical form【外形】 | Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 200 |
| conjugate【偶联物】 | alkaline phosphatase conjugate |
| antibody form【抗体形式】 | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous solution |
| species reactivity | mouse |
| technique(s) | direct ELISA: 1:15,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL) |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
