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Cathepsin L Activity Assay Kit
产品性质
| Quality Level【质量水平】 | 100 |
| web metadata keyword.default | Cathepsin L Activity Assay Kit|sigma-aldrich |
| Detection Method | fluorometric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | The kit is suitable for the measurement of cathepsin L in cell lysates. |
基本信息
| General description【一般描述】 | Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. |
| Features and Benefits【特点和优势】 | Cathepsin L Activity Assay Kit:
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| Principle【原理】 | The Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity. |
安全信息
| Pictograms【象形图】 | GHS05,GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H315 - H318 - H334 |
| Precautionary Statements | P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Classifications【危险分类】 | Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
