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DNAse I Activity Assay Kit (Fluorometric)
产品性质
Quality Level【质量水平】 | 100 |
web metadata keyword.default | DNAse I Activity Assay Kit (Fluorometric)|sigma-aldrich |
Detection Method | fluorometric |
storage temp.【储存温度】 | −20℃ |
suitability【适用性】 | The kit is suitable for the Measurement of DNAse I activity of purified proteins, the quantitative analysis of DNAse I mutants and inhibitors, and the comparative examination of DNAse I activity in serum and other biological samples. |
基本信息
General description【一般描述】 | Deoxyribonuclease I (DNAse I) is an endonuclease that cleaves DNA phosphodiester bonds yielding 5′-phosphorylated and 3′-hydroxylated oligonucleotides. DNAse I targets single-stranded DNA, double-stranded DNA, and chromatin in a non-specific manner. As an important player in cellular waste management, DNAse I is normally secreted extracellularly to clear the system from circulating cell-free DNA, foreign DNA from food digestion or potential pathogens, and endogenous chromosomal DNA from apoptotic and necrotic cells. Abnormal DNAse I activity occurs in association with a variety of cancers and auto-immune illnesses that exhibit elevated levels of cell-free DNA. Furthermore, DNAse I has been therapeutically used in cystic fibrosis patients to degrade DNA and reduce sputum viscosity. |
Features and Benefits【特点和优势】 | DNAse I Activity Assay Kit:
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Principle【原理】 | The DNAse I Activity Assay Kit allows for quantitative evaluation of DNAse I activity of purified enzymes and their inhibitors, as well as comparative examination of DNAse I activity in biological samples. Enzyme activity is detected upon cleavage of a DNA Probe, which yields a fluorescent DNA product measured at λEx = 651nm/λEm = 681nm. The limit of quantification (LOQ) is 178 fmoles of DNA probe cleaved per minute per mL. |
安全信息
Pictograms【象形图】 | GHS08 |
Signal word【警示用语:】 | Danger |
Hazard Statements | H334 |
Precautionary Statements | P261 - P284 - P304 + P340 + P312 - P501 |
Hazard Classifications【危险分类】 | Resp. Sens. 1 |
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |