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Gelatinase (Gelatin Degradation/Zymography) Assay Kit (Fluorometric)
产品性质
| usage【用途】 | sufficient for 100 fluorometric tests |
| Detection Method | fluorometric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | Suitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etc |
基本信息
| General description【一般描述】 | Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells. |
| Features and Benefits【特点和优势】 | Compatible with high-throughput handling systems. |
| Principle【原理】 | The Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU). |
安全信息
| Hazard Statements | H412 |
| Precautionary Statements | P273 - P501 |
| Hazard Classifications【危险分类】 | Aquatic Chronic 3 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
| Flash Point(F)【闪点(F)】 | 188.6 °F |
| Flash Point(C)【闪点(C)】 | 87 ℃ |
