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Pyruvate Assay Kit
| 产品编号： | 4072988 |
| 规格： | sufficient for 100 colorimetric or fluorometric tests |
| 包装规格： | 1 KIT |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| usage【用途】 | sufficient for 100 colorimetric or fluorometric tests |
| Detection Method | colorimetric fluorometric |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | Suitable for pyruvate detection in biological samples and for studying the effects of drugs on pyruvate metabolism. |
基本信息
| General description【一般描述】 | Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. |
| Features and Benefits【特点和优势】 | Compatible with high-throughput handling systems. |
| Principle【原理】 | The Pyruvate Assay Kit uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorometric assays. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
| Flash Point(F)【闪点(F)】 | 188.6 °F |
| Flash Point(C)【闪点(C)】 | 87 ℃ |
