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ReadyShield® Protease Inhibitor Cocktail
| 产品编号： | 4073014 |
| 规格： | for use in purification of Histidine-tagged proteins, Non-freezing solution |
| 包装规格： | 1 ML，5 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| description【描述】 | Purification of Histidine-Tagged Proteins |
| form【形式】 | (non-freezing liquid formulation ) |
| packaging【包装】 | pkg of 1 mL (in amber polypropylene vial) pkg of 5 mL (in amber polypropylene vial) |
| web metadata keyword.default | Extracts, Lysate, Cell Extracts, Tissue Extracts, Mammalian Tissue Extract, Bacterial lysate, Recombinant extracts, fusion proteins, Electrophoresis, peptidase inhibitor, proteinase inhibitor |
| storage temp.【储存温度】 | −20℃ |
基本信息
| Quality【质量】 | 200 |
| General description【一般描述】 | This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849. |
| Features and Benefits【特点和优势】 |
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| Preparation Note【制备说明】 | The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells. |
| Other Notes【其他说明】 | For life science research only. Not for use in diagnostic procedures. |
| Legal Information【法律信息】 | ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
