US-2019-nCoV Assays for detection of SARS-CoV-2 RNA

solution (in 10 mM Tris, 1 mM EDTA)

400

pkg of 3 vials

7.5-8.5

ambient

−20℃

US-2019-nCoV Assays are for qPCR (5′ nuclease assay) detection of SARS-CoV-2. The product is primers and probes, the sequences of which were designed and made publicly available by the United States Centers for Disease Control and Prevention (U.S. CDC).

For additional details, please visit SigmaAldrich.com/ncov.

The forward and reverse primers in each tube come with the following features:

• DNA bases
• No modifications
• Desalt purified
• 10 nmol each

The Dual-Labeled Probe in each tube comes with the following features:

• DNA bases
• 6-FAM^™ reporter
• BXQ (Block All Quencher—SigmaAldrich.com/bxq)
• HPLC purified
• 2.5 nmol

The product includes 3 individual tubes of 20X primer and probe pools for quantification of SARS-CoV-2 genes. Assays included are N1, N2, and RNase P (human). The forward primer, reverse primer, and probe are at final concentrations of 10, 10, and 2.5 μM, respectively.

N1 Assay:
Forward Primer GACCCCAAAATCAGCGAAAT
Reverse Primer TCTGGTTACTGCCAGTTGAATCTG
Dual-Labeled Probe ACCCCGCATTACGTTTGGTGGACC

N2 Assay:
Forward Primer TTACAAACATTGGCCGCAAA
Reverse Primer GCGCGACATTCCGAAGAA
Dual-Labeled Probe ACAATTTGCCCCCAGCGCTTCAG

RNase P Assay:
Forward Primer AGATTTGGACCTGCGAGCG
Reverse Primer GAGCGGCTGTCTCCACAAGT
Dual-Labeled Probe TTCTGACCTGAAGGCTCTGCGCG

FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

12 - Non Combustible Liquids