Anti-Actin (Ab-1) Mouse mAb (JLA20)

100

mouse

ascites fluid

primary antibodies

JLA20, monoclonal

liquid

≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)

all

Calbiochem^®

OK to freeze

IgM

wet ice

−20℃

1 ea in Plastic ampoule

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.

Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1

Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN

Entrez Gene Name: actin, alpha 1, skeletal muscle

Hu Entrez ID: 58, 71 (Related Antibodies: CP64)

Mu Entrez ID: 11459 ;-; 11465

Rat Entrez ID: 29437 ;-; 287876

Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.

Chicken
chicken cytoskeletal proteins

Immunoblotting (1:5000-1:20000)

Immunofluorescence (1:10000, see application references)



Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.

Following initial thaw, aliquot ascites and freeze (-20°C).

Positive Control
All animal cell lines

Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

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11 - Combustible Solids

WGK 1