Phorbol-12,13-dibutyrate-CAS 37558-16-0-Calbiochem

C₂₈H₄₀O₈

504.61

MFCD00036783

Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C (PKC). Stimulates the phosphorylation of Na⁺-K⁺ ATPase, thereby inhibiting its activity. Promotes the expression of iNOS in cultured hepatocytes. PDBu can be diluted to 30 µM in aqueous solutions, so it is commonly used in binding studies or in applications requiring high concentrations. It is easier to remove PDBu than PMA from cells in culture.
Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C. Stimulates the phosphorylation of Na⁺,K⁺- ATPase, thereby inhibiting its activity. Promotes the expression of inducible NOS in cultured hepatocytes. Commonly used in binding studies or in applications requiring high concentrations of phorbol compounds.

Product does not compete with ATP.
Primary Target
Na⁺,K⁺- ATPase
Reversible: no
Cell permeable: no

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Further dilute with aqueous buffers just prior to use.

Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427.
Lee, E.J., et al. 1994. Brain Res. 665, 127.
Critz, S.D., et al. 1993. J. Neurochem.60, 1175.
Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611.
Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥99% (HPLC)

solid (crystalline)

Calbiochem^®

OK to freeze

clear

DMSO: 10 mg/mL

ambient

−20℃

1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1

BQJRUJTZSGYBEZ-YVQNUNKESA-N

1, 5 mg in Alu drum
Packaged under inert gas

GHS05,GHS06,GHS08

Danger

H300 + H310 + H330 - H314 - H317 - H334 - H351

P201 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK 3