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Chondroitinase ABC from Proteus vulgaris

产品编号:4074275
规格:recombinant, low endotoxin, aqueous solution, ≥100 U/ml, 50-250 units/mg protein, BSA free
包装规格:0.1 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40742750.1 ML5520
产品性质
Quality Level【质量水平】
200
Specific Activity
50-250 units/mg protein
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
compatibility【相容性】
pH optimum:
  • pH 8.0 (chondroitin sulfate)
  • pH 6.8 (hyaluronic acid)

Temperature optimum: 37 ℃
Activator: 0.05 M acetate 
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+ 
基本信息
General description【一般描述】
Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.
Application【应用】
  • Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
  • Neural regeneration research
  • Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
  • Quantitation of glucosaminoglycans (mucopolysaccharides)
Features and Benefits【特点和优势】
  • Low Endotoxin
  • BSA Free
  • Recombinant
  • Endolytic component only, does not contain exolytic enzyme.
  • Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate
Components【组分】
Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water
Unit Definition【单位定义】
1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 ℃, using chondroitin sulfate from shark cartilage (C4384) as substrate.
Other Notes【其他说明】
This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.
产品说明
Storage and Stability【储存及稳定性】
Store the product at -70 ℃. When stored unopened at -70 ℃, the buffer is stable for at least two years.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
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