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Gecko2 Mouse Whole Genome CRISPR Pool, gRNA Only Lenti Particles (Gecko2 vector)
产品性质
| Quality Level【质量水平】 | 200 |
| packaging【包装】 | pkg of 8x25 μL (vials) |
| concentration【浓度】 | 5x108 VP/ml (via p24 assay) |
| application(s) | CRISPR |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
基本信息
| General description【一般描述】 | Developed by Feng Zhang′s lab at the Broad Institute, the mouse GeCKO v2 (two vector system) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using a dual lenti CRISPR vector wherein the pooled libraries will only express the gRNA (with the lentiGuide-Puro vector), this system produces over 100X higher titer virus compared to version 1. The lentiGuide-Puro pool should be used only in cell lines with Cas9 already integrated (which can be generated using a separate lenti-Cas9-Blast vector). Sigma′s lentiviral mouse GeCKO pool guide RNA only is provided in 8 x 25 ul aliquots at a minimum titer of 5X10^8 TU/ml (measured by a p24 assay). Each species-specific library is delivered as two half-libraries (A and B). It is recommended to screen both A and B libraries together, which will include 6 sgRNAs per gene (3 sgRNAs in each library). Both libraries contain 1000 non-targeting control sgRNAs. The A library also targets miRNAs (4 sgRNAs per miRNA). |
| Application【应用】 | Functional Genomics/Screening /Target Validation |
| Features and Benefits【特点和优势】 |
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| Preparation Note【制备说明】 | Puro Kill Curve and Determining CFU (Colony Formation Unit) per mL. Prior to performing a library-scale screening, two preliminary experiments must be conducted. Visit Sigma.com/pooledscreening. |
| Other Notes【其他说明】 | 该产品仅供研发使用，不可用作药物、家庭使用或其他用途。请查阅物质安全资料表，获取危险和安全操作规范信息。尽管生产的慢病毒转导颗粒是不可复制的，但仍然建议将其作为生物危害等级2级(RGL−2)生物体在实验室中进行处理。请遵循所有已发表的RGL-2指南，进行实验室废物处理和净化。 |
| Legal Information【法律信息】 | CRISPR专利正在申请中 |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
