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磷酸酶(碱性) 来源于小牛肠粘膜
Phosphatase, Alkaline from calf intestinal mucosa
产品价格查看更多规格...
| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 4075067 | 1 MG | 3610 | |||
| 4075067 | 5 MG | 12660 |
产品别名
9001-78-9
磷酸酶(碱性) 来源于小牛肠粘膜
Phosphatase, Alkaline from calf intestinal mucosa
Alkaline Phosphatase from calf intestinal mucosa
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.1.3.1 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-631-4 |
| MDL number | MFCD00131849 |
| eCl@ss | 42010105 |
| NACRES | NA.54 |
| Application【应用】 | Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays. |
| Biochem/physiol Actions【生化/生理作用】 | Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions. |
| Unit Definition【单位定义】 | 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 ℃ |
| Reconstitution【重悬】 | The solution can be used without dialysis (for enzyme immuno-assay). |
| Other Notes【其他说明】 | Sales restrictions may apply Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase |
产品性质
| form【形式】 | solution (clear, colorless) |
| Specific Activity | ~2500 U/mg protein (~10 mg/ml) |
| mol wt【分子量】 | Mr ~140000 |
| technique(s) | enzyme immunoassay: suitable |
| impurities【杂质】 | 0.1 mM ZnCl2 1 mM MgCl2 3 M NaCl 30 mM triethanolamine |
| pH【pH值(酸碱度)】 | ~7.6 |
| storage temp.【储存温度】 | 2-8℃ |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
