Transcreener^® GDP FP Assay

NA.84

The Transcreener^® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener^® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener^® GDP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of GDP and therefore is compatible with any enzyme that produces GDP, including GTPases such as CDC42. The Transcreener^® GDP Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to GTP concentration (1 to 1,000 μM GTP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and =85 polarization shift (mP) at 10% GTP conversion using 10 μM GTP.

The Transcreener^® GDP Assay was developed to follow the progress of any enzyme that produces GDP. The Transcreener^® GDP Detection Mixture comprises a GDP Alexa633 Tracer bound to a GDP Antibody. The tracer is displaced by GDP, the invariant product generated during the enzyme reaction. The displaced tracer freely rotates leading to a decrease in fluorescence polarization. Therefore, GDP production is proportional to a decrease in polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.

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3009-1K = 1,000 assay, 384-well
3009-10K = 10,000 assay, 384-well

Kit with buffered aqueous solutions

Transcreener is a registered trademark of BellBrook Labs

dry ice

−20℃