Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

200

mouse

purified antibody

secondary antibodies

HP6043, monoclonal

liquid

preservative

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG

wet ice

−20℃

500 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

Enzyme Immunoassay (1:1000, see application references)

Toxicity: Standard Handling (A)

In PBS, 10 mg/ml BSA.

Hamilton, R.G. 1993. Clin. Chem.39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.
Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG₁: 100%

Human IgG₂: 100%

Human IgG₃: 100%

Human IgG₄: 100%

Human IgM: <0.2%

Human IgA: <0.1%

Human IgE: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG₁: 100%

Human IgG₂: 100%

Human IgG₃: 100%

Human IgG₄: 100%

Human IgM: <0.01%

Human IgA: <0.01%

Human IgE: <0.01%



There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 2