MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli

≥90% (SDS-PAGE)

100

liquid

≥140 mU/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). Degrades fibrillar collagen Types I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13 resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
Note: 1 mU = 1 milliunit.
Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). The catalytic domain is comprised of amino acid residues Tyr⁸⁹ to Gly²⁶⁵ of mature human MT1-MMP. Hydrolyzes fibrillar collagens I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13, resulting in enhanced proteolysis of the matrix for cell migration and proliferation.

Toxicity: Standard Handling (A)

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Amano, T., et al. 2005. Cell Res.15, 150.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Sato, H., et al. 1994. Nature370, 61.
Knight, C.G., et al. 1992. REBS Lett.296, 263.

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