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产品性质
| biological source【生物来源】 | human skin |
| description【描述】 | Human melanoma, metastasis |
| growth mode【生长模式】 | Adherent |
| morphology【形态学】 | Epithelial |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Description【细胞系描述】 | Ma-Mel-5 (ESTDAB-149) was established from the cutaneous/subcutaneous metastasis in a 49 year-old female patient with metastatic melanoma (skin stage IV). The Ma-Mel-5 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission′s fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X, CSF1PO: 11,12 D13S317: 11,13 D16S539: 8,13 D5S818: 9,11 D7S820: 11 THO1: 9.3 TPOX: 8 vWA: 15,17 |
| Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37℃. Saturation density approx 15 x10,000 cells/cm² . |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information. |
