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Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment-Alkaline Phosphatase antibody produced in goat
| 产品编号： | 4078330 |
| 规格： | affinity isolated antibody, buffered aqueous glycerol solution |
| 包装规格： | 0.25 ML，0.5 ML，1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD00162782 |
| NACRES | NA.46 |
| General description【一般描述】 | IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-Alkaline Phosphatase antibody binds all rabbit immunoglobulins. |
| Immunogen【免疫原】 | Purified rabbit IgG |
| Application【应用】 | Western blot analysis of prostate epithelial cell lysates was performed using alkaline phosphatase conjugated goat anti-rabbit F2 Fragment specific antibody as the secondary at a dilution of 1:7500 for 2 hours at room temperature. Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Enzyme-linked immunosorbent assay (1 paper) Western Blotting (1 paper) Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-Alkaline Phosphatase antibody is suitable for use in immunoblotting (5μl) and direct ELISA (1:30,000). The product may also be used for immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections). |
| Physical form【外形】 | Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | goat |
| conjugate【偶联物】 | alkaline phosphatase conjugate |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | buffered aqueous glycerol solution |
| species reactivity | rabbit |
| technique(s) | direct ELISA: 1:30,000 dot blot: 1:30,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 western blot: 1:30,000 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
