ESGRO Complete Accutase

32160801

NA.75

ESGRO Complete^™ Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium (Cat. No. SF001-500). Accutase does not contain mammalian or bacterial derived products.

Cell Detachment:

1. Thaw Accutase^® to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.

Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase^® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

100

liquid

Chemicon^®

cell culture | stem cell: suitable

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

dry ice

Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase^® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1