Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

100

mouse

purified antibody

primary antibodies

7E3-1B8, monoclonal

liquid

≤0.1% sodium azide as preservative

rat

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG2a

wet ice

−20℃

100 μL in Glass bottle
Please refer to vial label for lot-specific concentration.

Recognizes the ~95 kDa PSD95 protein.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.
This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Rat
recombinant, rat PSD95

Immunoblotting (1:2000)
Immunofluorescence (1:200, indirect)
Immunoprecipitation (see comments)

Toxicity: Standard Handling (A)

In PBS.

Following initial thaw, aliquot and freeze (-20°C).

Immunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95′s proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Brakeman, P.R., et al. 1997. Nature386, 284.
Hualing, D., et al. 1997. Nature386, 279.
Kennedy, M.B. 1997. Trends in Neurosci.20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol.7, 93.
Cho., K.-O., et al. 1992. Neuron9, 929.

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11 - Combustible Solids

WGK 1