Amelogenin, human

expressed in E. coli

wet ice

−20℃

Amelogenin, X isoform belongs to the family of closely related amelogenin proteins that participate in the amelogenesis process of teeth mineralization. Amelogenins account for more than 90% of the total protein in developing tooth enamel. Mutations in AMELX are responsible for development of the rare disease Amelogenesis imperfecta 1E (AI1E), which is characterized by abnormal tooth enamel development. Amelogenins act both as attachment factors and as a growth factor for mesenchymal stem cells. Amelogenin is of increasing importance as a potential therapeutic agent for variety of application including treatment hard-to-heal wounds, promoting remineralization of caries lesions, repairing human tooth enamel.
This recombinant product expressed in E. coli has the protein sequence below and does not contain any added sequence tags:
MPLPPHPGHPGYINFSYEVLTPLKWYQSIRPPYPSYGYEPMGGWLHHQIIPVLSQQHPPTHTLQPHHHIPVVPAQQPVIPQQPMMPVPGQHSMTPIQHHQPNLPPPAQQPYQPQPVQPQPHQPMQPQPPVHPMQPLPPQPPLPPMFPMQPLPPMLPDLTLEAWPSTDKTKREEVD
The product is supplied as lyophilized powder, prepared from a 0.2μM filtered solution of Amelogenin in 2% acetic acid without any additives. The lyophilized product is essentially salt free.
The biological activity of recombinant, human AMELX is measured by its ability to promote attachment of Saos-2 cells to a coated surface. The ED50 is defined as the amount of AMELX per cm² that elicits 50% of the maximal attachment activity.

The product can be reconstituted in water or 2% acetic acid in a concentration of 0.1-5 mg/ml. Tap vial to make sure protein is reconstituted. Aliquot reconstituted Amelogenin and store at -20 ℃. Avoid repeated freeze/thaw cycles. Amelogenin may form thin clear film on some surfaces, leading to reduced concentration in solution. Exact Amelogenin concentration can be determined by absorption at 280nm. Amelogenin solution of 0.1% has A₂₈₀=1.35

11 - Combustible Solids

WGK 3