Yeast and Mold Selective Broth

32160801

NA.74

The microbiology of beverages will vary greatly depending upon the method of processing and the means of preservation. High microbial populations often indicate poor quality in raw material, unsanitary equipments or opportunity for growth in the food at some stage in the process. Yeast and Mould Selective Broth is used for the detection of fungi in routine analysis of beverages using membrane filter technique.(1) This medium is used for enrichment of yeasts and moulds from populations containing bacteria. The medium is highly nutritious for the growth of yeasts and moulds. Biopeptone and yeast extract provide nitrogenous compounds and vitamin B complex. Thiamine is also a B vitamin in the medium. Dextrose acts as the energy source. Diastase is a mixture of amylolytic enzymes. Bromocresol green is the pH indicator, which is green at acidic pH (pH 4.0) while blue at pH 5.6. Potassium phosphate helps in maintaining buffering action in the medium. The low pH inhibits bacterial growth. yeast produces green, opaque large colonies. Mold colonies are green and filamentous

Yeast and Mould Selective Broth is used for testing soft drinks and other beverages for growth of yeast and mold. Antibiotics in the medium prevent bacterial overgrowth in samples such as Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Incubation Time: 48–72 hrs
Incubation Temperature: 28–32 °C

酵母菌和霉菌选择性培养基

QC organisms
酿酒酵母 (ATCC 7754)

A. niger (ATCC 16404)
Target Microorganisms: Yeast and mold (selection by antibiotics)
酵母菌落显示为绿色和不透明的大菌落。 霉菌菌落为绿色和细丝状。

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

100

liquid

12 mo.

ready-to-use

green

sample type bottled water
sample type cider
sample type food(s)
sample type beverage(s)
sample type soft drink
sample type beverage(s)
sample type cosmetics
sample type beer
sample type wine
sample type sport drink

4.4±0.2 (25.0 ℃)

clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
microbiology

for use with MF-method
for use with Microfil^®
for use with MicropreSure^®
for use with Milliflex^®

wet ice

2-8℃

50 pk in 2 mL plastic ampoules
2mL 塑料安瓿

12 - Non Combustible Liquids

WGK 1