KAPA2G Fast PCR Kit

NA.54

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

KAPA2G Fast PCR Kit has been used in:

• PCR with confronting 2 -pair primers assay (PCR-CTPP)
• Routine PCR
• Fast PCR
• Genotyping

KAPA2G Fast DNA Polymerase generated DNA fragments is useful in sequencing restriction enzyme digestion and cloning experiments. KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities like wild-type Taq, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 10⁵ nucleotides incorporated.

Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4℃ is not recommended. Please note that reagents stored at temperatures above -20℃ are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

For Research Use Only. Not for use in diagnostic procedures.

组份不可单独销售
KAPA2G Fast Standard or HotStart^® DNA Polymerase (5 U/μL)
5X KAPA2G Buffer A with MgCl₂
MgCl₂ 25 mM

100

sufficient for 500 reactions

≤18 mo.

Fast PCR
Multiplex PCR
dNTPs included
hotstart: no

pkg of 100 U (KK5008)
pkg of 250 U (KK5009)

Roche

PCR: suitable

purified DNA

dry ice

−20℃

GHS07,GHS08

Warning

H302 - H371 - H412

P260 - P264 - P270 - P273 - P308 + P311 - P501

Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2

12 - Non Combustible Liquids

WGK 1