Anti-Rabbit IgG F(ab′)₂-Rhodamine antibody produced in goat

NA.46

Immunoglobulin G (IgG) is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. It belongs to the immunoglobulin family and is a widely expressed serum antibody, consisting of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.
Immunoglobulin G (IgG) possesses two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. This antibody belongs to the immunoglobulin family and is a widely expressed serum antibody. The monomeric 150kDa structure of IgG constitutesIgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab′)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c).

Rabbit IgG F(ab′)2 fragment

Anti-Rabbit IgG F(ab′)2-Rhodamine antibody produced in goat has been used for immunofluorescence.
Anti-Rabbit IgG F(ab′)2-Rhodamine antibody produced in goat has been used in immunofluorescence.

Antibody format: IgG

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitute with 1.0 mL deionized water (or equivalent).

100

goat

rhodamine conjugate

affinity isolated antibody

secondary antibodies

polyclonal

lyophilized powder

rabbit

direct immunofluorescence: suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable

wet ice

2-8℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS06,GHS09

Danger

H302 - H311 - H411

P280 - P301 + P312 + P330 - P302 + P352 + P312

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2

EUH032

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK 3