Monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody produced in mouse

NA.46

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.
Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

bovine IgG1 and IgG2

Purified bovine IgG

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunohistochemistry

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

200

mouse

alkaline phosphatase conjugate

purified immunoglobulin

secondary antibodies

BG-18, monoclonal

buffered aqueous glycerol solution

bovine (non-reactive to IgM)

goat, canine, horse, pig, sheep, human, rabbit, cat, guinea pig

direct ELISA: 1:25,000
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
western blot (chemiluminescent): 1:100,000

IgG1

wet ice

2-8℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)