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产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
4081399 | 25 MU | 7420 |
产品别名
9002-07-7
Trypsin, Bovine Pancreas
基本信息
Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.21.4 ( BRENDA | IUBMB ) |
General description【一般描述】 | Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8. Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units. Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units. |
Warning【警告】 | Toxicity: Irritant (B) |
Unit Definition【单位定义】 | One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6. |
Physical form【外形】 | Salt-free |
Reconstitution【重悬】 | Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C. |
Other Notes【其他说明】 | Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17. Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799. Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
form【形式】 | solid |
Specific Activity | ≥2,500 USP units/mg dry wt |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze desiccated (hygroscopic) |
solubility【溶解性】 | HCl: 1 mM neutral buffer: soluble water: soluble |
foreign activity【异质活性】 | Chymotrypsin ≤50 NF units/mg dry wt. |
shipped in【运输】 | ambient |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 25 mu in Plastic ampoule 2.5 mu in Glass bottle |
安全信息
Pictograms【象形图】 | GHS07,GHS08 |
Signal word【警示用语:】 | Danger |
Hazard Statements | H315 - H319 - H334 - H335 |
Precautionary Statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
Hazard Classifications【危险分类】 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
Target Organs【靶器官】 | Respiratory system |
Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
WGK | WGK 1 |